Affiliation:
1. Nanjing Forestry University
2. Nanjing University of Aeronautics and Astronautics
3. Southeast University
Abstract
Prion protein has drawn great attention due to its pathological potential to prion diseases. Discriminate and detection of the trace quantities PrPSc is an important measure for prion disease diagnosis at the presymptomatic stage. In this study, we developed a novel sandwich surface plasmon resonance (SPR) assay for the detection of PrPSc, involving bare gold surfaces and bare gold nanoparticles. PrPSc can be captured by the SPR sensing surface via a surface assisted coupling reaction between its intra-molecular disulfide bond and the gold atoms, while PrPC cannot bind to the gold surface strongly. The gold nanoparticles were proved to amplify the SPR detection signals via the coupling of their localized surface plasmon (LSP) with the propagating plasmon on the SPR gold surface. Our results confirmed that the bare SPR gold surface successfully captured the PrPSc from the solution with a LOD of 0.5ng/mL and a linear detection range from 0.5ng/mL to 500ng/mL. Injecting the gold nanoparticles after PrPSc yielded a dramatic enhancement of signal, with a lower LOD of 0.001ng/mL and a linear detection range from 0.001ng/mL to 10ng/mL. The gold nanoparticles permitted 4 to 322-fold increase of the signals. The required detection time was controlled in 15 min. PrPC, cys-protein G and their mixtures with PrPSc, were also detected via this sandwich SPR detection assay. Atomic force microscope (AFM) was used to evaluate the surface morphology of the SPR gold substrate after the detection. All the obtained results suggested that this novel SPR sandwich detection assay in our work was efficient, sensitive and specific for the detection of trace PrPSc
Publisher
Trans Tech Publications, Ltd.
Cited by
3 articles.
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