Affiliation:
1. Islamic Azad University
Abstract
Glucose oxidase sensor is one of the best methods for measuring small amounts of glucose; this sensor can be modified using colloidal gold nanoparticles as complementary substance in the electrode structure with the aim of increasing efficiency. In this article, the colloidal gold nanoparticles (24 nm) were used to construct the two electrodes. The first electrode (Au/CP) was constructed of 100 mg carbon graphite powder, 36 µl paraffin oil and 300 µl colloidal gold nanoparticles. The second electrode (Au/CILE) was constructed of 100 mg carbon graphite powder, 36 µl paraffin oil, 300 µl colloidal gold and 50 mg ionic liquid oppf6. To evaluate the performance of each electrode and to prepare of bio sensor, these were covered by solution of 10 mg glucose oxidase and 1 ml phosphate buffer. The bio sensors were test with 0.8 mM glucose solution. By comparing the amount of AMP and the transfer time for both, the according results, the effect of colloidal gold nanoparticles on the increasing conductivity, sensitivity and stability biosensor, was determined.
Publisher
Trans Tech Publications, Ltd.
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