Effect of the Lactide/Glycolide Ratio and Molecular Weight of PLGA on the Bovine BMPs Microspheres Systems

Abstract

The present investigation was aimed at optimization of BMPs loaded PLGA microspheres formulations resulting in improved encapsulation efficiency and sustained release of BMPs by varying the molecular weight and copolymer composition of PLGA. Double-emulsion solvent evaporation method was used to prepare the microspheres. The effect of polymer molecular weight and copolymer composition on particle properties and release behavior in vitro was reported. The particle size and encapsulation efficiency increased with increase in molecular weight and lactide content of PLGA. While BMPs release in vitro decreased with increase in molecular weight and lactide content of PLGA. SEM pictures revealed that almost all microspheres were spherical but internal morphology was different. The morphology of PLGA microspheres with exorbitant molecular weight(100kD) was anomalistic whereas the morphology of PLGA microspheres with higher glycolide content(50) have porous structures.