Abstract
Selenium (Se) and zinc (Zn) are essential animal microminerals. Combining Se and Zn (Se-Zn) as a feed additive in its influence on rumen fermentation patterns is still very limited, so further investigation is needed. The present study explored the supplementation impact of combined Se-Zn from organic sources on rumen enzyme activity and relative abundance of several bacterial species through an in vitro method. Five treatments, each with six replicates were used in the study. The first group treated without Se and Zn supplementation (T0, control), the second group treated with 0.3 ppm Se + 60 ppm Zn (T1), the third group treated with 0.45 ppm Se + 60 ppm Zn (T2), the fourth group treated with 0.3 ppm Se + 90 ppm Zn (T3), and the fifth group treated with 0.45 ppm Se + 90 ppm Zn (T4). The parameters observed included rumen microbial enzyme activities (carboxyl methyl cellulase, amylase, protease) and the relative abundance of rumen microbes (Ruminococcus sp., Ruminococcus flavefaciens, Ruminococcus albus, Streptococcus sp., Prevotella ruminicola, and Eubacterium ruminantium). Results indicated that carboxyl methyl cellulase (CMC-ase) and amylase activities raised in T2, T3, and T4 in comparison to T1 and T0 treatments. Protease activity and protein enzyme content increased in T2 compared to all treatments. The relative abundance of Ruminococcus sp. and Ruminococcus albus was higher in T2 and T3 compared to T0 treatment. Furthermore, an elevated Ruminococcus flavefaciens was indicated in T2 compared to other treatments. The T2, T3, and T4 led to higher abundances of Eubacterium ruminantium, Prevotella ruminicola, and Ruminococcus albus compared to T0 and T1. It is concluded that organic Se and Zn enhanced the relative abundance of several bacterial species and the activity of enzymes in the rumen; optimal results are recommended when combining 0.45 ppm Se + 60 ppm Zn.
Reference33 articles.
1. Al-Taie YAM and Almahdawi MKK (2021). Effect of selenium and zinc doses and interaction between them on production performance and some carcass traits of Awassi lambs. Annals of the Romanian Society for Cell Biology, 25(4): 2506-2520. Available at: https://annalsofrscb.ro/index.php/journal/article/view/2782
2. Anam MS, Astuti A, Widyobroto BP, Gunawan, and Agus A (2023). In vitro ruminal cumulative gas and methane production, enzyme activity, fermentation profile and nutrient digestibility on feed supplemented with organic selenium. American Journal of Animal and Veterinary Sciences, 18(4): 261-272. DOI: https://www.doi.org/10.3844/ajavsp.2023.261.272
3. Association of official analytical chemists (AOAC) (2005). Official methods of analysis, 18th Edition. Arlington., Washington, DC, USA.
4. Effect of zinc sources on milk yield, milk composition and plasma concentration of metabolites in dairy cows;Bakhshizadeh;South African Journal of Animal Science 49(5),2019
5. Effects of prepartum zinc-methionine supplementation on feed digestibility, rumen fermentation patterns, immunity status, and passive transfer of immunity in dairy cows;Chen;Journal of Dairy Science 103(10),2020