Abstract
The results of molecular genetic analysis of 13 polymorphic variants of genes, according to the data of genome-wide association studies (GWAS) associated with the development of addictive states, in the group of persons with alcohol dependence (n = 142) and in the comparison group (n = 211) are presented. The main genotyping method is polymerase chain reaction (PCR) followed by high resolution melting (HRM) and clustering of melting profiles; the melting results are validated using the restriction fragment length polymorphism (PCR-RFLP) method. As a result, single nucleotide polymorphisms associated with an increased likelihood of alcohol dependence are genotype GG (rs7085104, AS3MT); genotype GG (rs7590720, PECR); allele C (rs11191580, NT5C2); allele T (rs17504622); allele A (rs73229090, EPHX2); allele A (rs1109501, MUC7 ).
Publisher
Belarusian State University
Reference42 articles.
1. Shield KD, Rylett M, Rehm J. Public health successes and missed opportunities. Trends in alcohol consumption and attributable mortality in the WHO European Region, 1990–2014. Copenhagen: World Health Organization Regional Office for Europe; 2016. 88 p.
2. Kumar R, Kumar KJ, Benegal V. Trait impulsivity in alcohol-naive offspring at high risk for alcoholism. Indian Journal of Psychological Medicine. 2018;40(6):547–555. DOI: 10.4103/IJPSYM.IJPSYM_92_18.
3. Dawson DA. The link between family history and early onset alcoholism: earlier initiation of drinking or more rapid development of dependence? Journal of Studies on Alcohol. 2000;61(5):637–646. DOI: 10.15288/jsa.2000.61.637.
4. Moss HB, Chen CM, Yi H-Y. Subtypes of alcohol dependence in a nationally representative sample. Drug and Alcohol Dependence. 2007;91(2–3):149–158. DOI: 10.1016/j.drugalcdep.2007.05.016.
5. Brennan P, Lewis S, Hashibe M, Bell DA, Boffetta P, Bouchardy C, et al. Pooled analysis of alcohol dehydrogenase genotypes and head and neck cancer: a HuGE review. American Journal of Epidemiology. 2004;159(1):1–16. DOI: 10.1093/aje/kwh003.