Study of the efficiency of suppressing fire blight by bacteriophages under laboratory conditions-Reference-Cited by-同舟云学术

Study of the efficiency of suppressing fire blight by bacteriophages under laboratory conditions

Published:2022-02-21 Issue:1 Volume: Page:59-69
ISSN:2957-5079
Container-title:Experimental Biology and Biotechnology
language:
Short-container-title:Experimental Biology and Biotechnology

Author:

Besarab Natalya V.^ORCID,Lagonenko Alexander L.^ORCID,Letarova Maria A.,Golomidova Alla K.,Kulikov Evgeny E.^ORCID,Letarov Andrey V.^ORCID,Evtushenkov Anatoly N.^ORCID

Abstract

Erwinia amylovora bacteriophages Hena2, Roscha1, Pixel, Dichka and VyarbaS were studied as a biological fire blight control agents on model objects – pear flowers and leaves. When treated with a bacteriophage 1 h before inoculation with E. amylovora 1/79Sm, no statistically significant differences in symptoms were found compared to the control. However, a decrease in the titer of the pathogen by 1–3 orders of magnitude was shown with E. amylovora in the inoculum of 106 –108 CFU/mL and treatment with a bacteriophage 1 h before infection with the pathogen.

Publisher

Belarusian State University

Reference24 articles.

1. Vrancken K, Holtappels M, Schoofs H, Deckers T, Valcke R. Pathogenicity and infection strategies of the fire blight pathogen Erwinia amylovora in Rosaceae: state of the art. Microbiology. 2013;159(part 5):823–832. DOI: 10.1099/mic.0.064881-0.

2. Johnson KB. Fire blight of apple and pear. The Plant Health Instructor [Internet]. 2000 [cited 2021 August 24]. Available from: https://www.apsnet.org/edcenter/disandpath/prokaryote/pdlessons/Pages/FireBlight.aspx. DOI: 10.1094/PHI-I-2000-0726-01.

3. Bogs J, Bruchmüller I, Erbar C, Geider K. Colonization of host plants by the fire blight pathogen Erwinia amylovora marked with genes for bioluminescence and fluorescence. Phytopathology. 1998;88(5):416–421. DOI: 10.1094/PHYTO.1998.88.5.416.

4. Momol MT, Norelli JL, Piccioni DE, Momol EA, Gustafson HL, Cummins JN, et al. Internal movement of Erwinia amylovora through symptomless apple scion tissues into the rootstock. Plant Disease. 1998;82(6):646–650. DOI: 10.1094/PDIS.1998.82.6.646.

5. Santander RD, Català-Senent JF, Figàs-Segura À, Biosca EG. From the roots to the stem: unveiling pear root colonization and infection pathways by Erwinia amylovora. FEMS Microbiology Ecology. 2020;96(12):fiaa210. DOI: 10.1093/femsec/fiaa210.