Preservation by Desiccation of Isolated Rat Hearts for 48 Hours Using Carbon Monoxide (PCO = 4,000 hPa) and Oxygen (PO2 = 3,000 hPa)

Author:

Hatayama Naoyuki1,Naito Munekazu1,Hirai Shuichi1,Yoshida Yu2,Kojima Tomohiro2,Seki Kunihiro3,Li Xiao-Kang4,Itoh Masahiro1

Affiliation:

1. Department of Anatomy, Tokyo Medical University, Tokyo, Japan

2. Resonance Club Co., Tokyo, Japan

3. Faculty of Science, Kanagawa University, Kanagawa, Japan

4. National Research Institute for Child Health and Development, Tokyo, Japan

Abstract

It is currently said that CO has anti-inflammatory and antiapoptosis effects and it has attracted attention as a medical gas. We used CO for rat hearts and conducted a preservation experiment. We isolated rat hearts, placed them into a specially made chamber, filled the chamber with a gas mixture of PCO (4,000 hPa) and PO2 (3,000 hPa), and preserved the hearts in a refrigerator at 4°C for 48 h. We then performed a heterotrophic transplantation on the neck of each recipient rat and resuscitated the preserved hearts. We herein report our findings.

Publisher

SAGE Publications

Subject

Transplantation,Cell Biology,Biomedical Engineering

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