Preliminary Study of β1-Adrenergic Receptor and M2 Muscarinic Cholinergic Receptor Mediated Protective Effect of Electroacupuncture on PC6 against Myocardial Ischemia

Author:

Li Shi Candidate,Zhao Ji-Ping,Gao Jun-Hong,Zhang Jin-Ling,Zhu Bing,Yu Xiao-Chun,Rong Pei-Jing

Abstract

The aims of the present study were to observe the effect of electroacupuncture (EA) at Neiguan (PC6) on ischemic myocardium and the involvement of myocardial β1-adrenergic (β1AR) and M2 cholinergic muscarinic receptors (M2AChR) in the protective effect. The C57BL6 mice were divided into control, model and PC6 groups randomly. The myocardial ischemia (MI) model of mice was established by forced exhaustive swimming (ES). EA at PC6 was performed to the PC6 group for 7 days. The Electrocardiograph (ECG) was recorded before ES and immediately after ES on the 1st and 7th day. The ECG J-point deviation value and heart rate (HR) were analyzed to evaluate the effect of EA. The β1AR and M2AChR knockout mice were used to explore whether β1AR and M2AChR in the cardiac myocytes mediated the cardioprotection effect of EA. The β1AR knockout (β1AR-/-) and M2AChR knockout (M2AChR-/-) mice were divided into MI model and PC6 groups respectively and treated the same way corresponding to the groups of C57BL6 mice. The results showed that the Electrocardiograph (ECG) J-point deviation value in C57BL6 mice were significantly increased after MI (P=0.015 in the model group, P=0.001 in the PC6 group), which is a symptom of MI, while the heart rate (HR) on the 1st decreased significantly (P<0.05, or P<0.01). Following EA in the C57BL6 mice, the J-point deviation on the 7th day was significantly decreased (P=0.031, decreased 70.05%), but the HR was still much slower than their baseline (both P<0.01). The increased J-point deviation value and decreased HR level were also observed in both β1AR-/- and M2AChR-/- mice (P<0.05, or P<0.01). While no significant changes were found in the J point deviation after EA intervention on the 7th day, and in HR of the both types of mice. These results suggested that EA at PC6 could protect the myocardium against ischemic injury, probably mediated by myocardial β1AR and M2AChR.

Publisher

Cognizant, LLC

Subject

Anesthesiology and Pain Medicine,Complementary and alternative medicine,General Neuroscience

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3