Affiliation:
1. Department of Biomedical Laboratory Science, Korea Nazarene University, Cheonan, Korea
Abstract
Previously, it was reported that the cotransfection of angiostatin K1-3, endostatin, and saxatilin genes using cationic liposomes significantly inhibited tumor progression. IL-12 is a well-known immune modulator that promotes Th1-type antitumor immune responses and also induces antiangiogenic
effects. In this study, we have examined the antitumoral function of the IL-12 gene cotransfected with antiangiogenic genes for angiostatin K1-3, endostatin, and saxatilin by O,O′-dimyristyl-N-lysyl glutamate (DMKE) cationic liposomes in a mouse tumor model. According to
our results, the administration of the IL-12 gene or the genes for angiostatin K1-3, endostatin, and saxatilin exhibited effective inhibition of B16BL6 melanoma growth in mice. In particular, intravenous administration of the IL-12 gene along with intratumoral administration of the three antiangiogenic
genes synergistically inhibited the B16BL6 tumor growth. These results suggest that systemically expressed IL-12 enhances antitumoral efficacy of locally expressed antiangiogenic proteins.
Subject
Cancer Research,Oncology,General Medicine
Reference30 articles.
1. Cationic lipid-DNA complexes for non-viral gene therapy: Relating supramolecular structures to cellular pathways;Expert Opin. Biol. Ther.,2005
2. Nonviral vectors in the new millennium: Delivery barriers in gene transfer;Hum. Gene Ther.,2001
3. In vitro and in vivo gene-transferring characteristics of novel cationic lipids, DMKD (O,O′-dimyristyl-N-lysyl aspartate) and DMKE (O,O′-dimyristyl-N-lysyl glutamate);J. Control. Release,2006
4. Synergy between angiostatin and endostatin: Inhibition of ovarian cancer growth;Cancer Res.,2000
5. Inhibition of angiogenesis and tumor progression by hydrodynamic cotransfection of angiostatin K1-3, endostatin, and saxatilin genes;Cancer Gene Ther.,2006
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献