Affiliation:
1. Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, P.R. China
2. Department of Oncology, The Second People’s Hospital of Taizhou, Taizhou, Jiangsu Province, P.R. China
Abstract
We aimed to investigate the significant role of long noncoding RNA X inactive specific transcript (XIST) in regulating tumor metastasis in colorectal cancer (CRC), as well as its possible mechanism. Expression of lncRNA XIST in CRC tissues and CRC cells was detected. CRC cells were
transfected with pc-XIST, blank control si-XIST, or si-control, and then the effects of lncRNA XIST on CRC cell migration and invasion were investigated, along with the interaction between lncRNA XIST and miR-137. lncRNA XIST was upregulated in CRC tissues. Compared with HT29 cells that had
low metastatic potential, XIST was markedly more highly expressed in LoVo cells that had a higher metastatic potential. Overexpression of XIST promoted the migratory and invasive potential of HT29 cells, while knockdown of XIST inhibited the migratory and invasive potential of LoVo cells.
Moreover, epithelial‐mesenchymal transition (EMT) markers, including E-cadherin, N-cadherin, and vimentin, exhibited corresponding expression changes. In addition, miR-137 was inhibited by XIST, and inhibition of miR-137 could reverse the effects of knockdown of XIST on the migratory
and invasive potential of LoVo cells. Furthermore, enhancer of zeste homolog 2 (EZH2) was confirmed as a target of miR-137. Our data reveal that lncRNA XIST may promote tumor metastasis in CRC possibly through regulating the miR-137‐EZH2 axis. lncRNA XIST may serve as a prognostic indicator
for CRC progression.
Subject
Cancer Research,Oncology,General Medicine
Reference34 articles.
1. Colorectal cancer statistics, 2017;CA Cancer J Clin.,2017
2. Cancer treatment and survivorship statistics, 2016;CA Cancer J Clin.,2016
3. Analysis of expression of cyclin E, p27kip1 and Ki67 protein in colorectal cancer tissues and its value for diagnosis, treatment and prognosis of disease;Eur Rev Med Pharmacol Sci.,2016
4. Colorectal cancer molecular biology moves into clinical practice;Gut,2011
5. The hallmarks of cancer: A long non-coding RNA point of view;RNA Biol.,2012
Cited by
53 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献