Abstract
The in vitro models have great potential in skin-related research as well as in testing for active ingredients in cosmetics, dermocosmetics and pharmaceuticals. Human skin behavior can be simulated in vitro using a variety of methods ranging from cell monolayer models to complicated organotypic and bioengineered three-dimensional models. Moreover, skin in vitro models offer an excellent alternative to animal testing in cosmetics and some of them are validated to be used as preclinical as-says. However, the in vitro simulation of the whole skin together with its appendages is still in its early stages. In this article we discuss a short evolution of skin models with its challenges and its future.