SUBLETHAL EFFECTS OF IMIDACLOPRID ON HAEMATOLOGICAL AND BIOCHEMICAL PROFILE OF FRESHWATER FISH, CYPRINUS CARPIO

Abstract

Imidacloprid is a commercialized Neonicotinoid pesticide, widely used to kill piercing and sucking types of insect pests in agriculture field. Due to its systemic nature and high water solubility, imidacloprid gradually reach and contaminate surrounding water bodies. The present study was planned to investigate the sublethal toxic impacts of pesticide imidacloprid on haematological and biochemical profile of freshwater fish, Cyprinus carpio. The fingerlings of Cyprinus carpio were exposed to three different sublethal concentrations of imidacloprid (7.8 ppm, 15.6 ppm and 23.4 ppm) for 30 days. Blood samples from all treatments were collected and used for hematological as well as biochemical diagnosis. During the trial period, haematological parameters like TEC, Hb and Hct were decreased significantly (P<0.05), whereas TLC count was found to be increased in imidacloprid treated fish. Similarly MCH count was lower but MCV was higher significantly as compared to control. In the biochemical analysis, plasma protein levels were significantly  decreased in all the treatment groups when compared to control. Similar trends of significant decrease in the values of albumin and globulin was also reported. Thus, results of present study show that imidacloprid can cause remarkable alterations in the haematological and biochemical parameters of Cyprinus carpio.