Frequency of red blood cells antibodies in possible cases of hemolytic disease of the newborn laboratory evidenced at Hemopa

Abstract

Purpose: To describe the frequency of red blood cells antibodies possibly related to Hemolytic Disease of the Newborn cases reported laboratory evidenced at Fundação Centro de Hemoterapia e Hematologia do Pará. Methods: This is a descriptive, cross-sectional study, based on the analysis of immuno-hematological reports of newborns filed at the Immunohematology Laboratory of the Fundação Centro de Hemoterapia e Hematologia do Pará , released between January 2015 and December 2019. The obtained data are related to ABO/Rh(D) typing; Direct Antiglobulin test; Irregular Antibodies test; Eluate, which were transcribed to Microsoft Excel sheets for later descriptive analysis. The study was approved by the Research Ethics Committee of the Fundação Pública Estadual do Hospital das Clínicas Gaspar Vianna, under the legal opinion #3,435,869. Results: A total of 37 reports were analyzed, laboratory evidence showed 34 cases of positive Direct Antiglobulin Test, of these, 22 presented positive eluate test. The highest frequency of described cases was related to anti-D antibody (19/22; 86.4%), followed by anti-c (1/22; 4.5%) and clinically significant antibody associations (2/22; 9.1%). Conclusion: The most frequent alloantibody evidenced was anti-D, however, other red blood cells alloantibodies observed drew attention and were considered relevant scientific evidence regarding the subject.