Affiliation:
1. The Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark
2. The Department of Biology, University of Copenhagen, Copenhagen, Denmark
Abstract
The vast majority of a bacterial population is killed when treated with a lethal concentration of antibiotics. The time scale of this killing is often comparable with the bacterial generation time before the addition of antibiotics. Yet, a small subpopulation typically survives for an extended period. However, the long-term killing dynamics of bacterial cells has not been fully quantified even in well-controlled laboratory conditions. We constructed a week-long killing assay and followed the survival fraction of Escherichia coli K12 exposed to a high concentration of ciprofloxacin. We found that long-term survivors were formed during exponential growth, with some cells surviving at least 7 d. The long-term dynamics contained at least three time scales, which greatly enhances predictions of the population survival time compared with the biphasic extrapolation from the short-term behavior. Furthermore, we observed a long memory effect of a brief starvation pulse, which was dependent on the (p)ppGpp synthase relA. Specifically, 1 h of carbon starvation before antibiotics exposure increased the surviving fraction by nearly 100-fold even after 4 d of ciprofloxacin treatment.
Funder
Danish National Research Foundation
Independent Research Fund Denmark
Villum foundation
Publisher
Life Science Alliance, LLC
Subject
Health, Toxicology and Mutagenesis,Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Ecology
Reference44 articles.
1. Pezzani MD , Be G , Cattaneo P , Zaffagnini A , Gobbi F , Rodari P , Bisoffi Z , Tacconelli E (2019) Evidence based review on optimal duration of antibiotic therapy for bacterial infections to support antimicrobial stewardship recommendations WHO Secretariat Nicola Magrini, Secretary of the Expert Committee on Selection and Use of Essential Medicines 1–28. https://www.who.int/selection_medicines/committees/expert/22/applications/ABWG_optimal_duration_AB.pdf
2. TREATMENT OF STAPHYLOCOCCAL INFECTIONS WITH PENICILLIN BY INTERMITTENT STERILISATION
3. Bacterial Persistence as a Phenotypic Switch
4. Definitions and guidelines for research on antibiotic persistence
5. Raising the flag on marine alien fouling species
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