Affiliation:
1. Institute of Innovative Research, Tokyo Institute of Technology
Abstract
TheBRCA2tumor suppressor plays a critical role in homologous recombination by regulating RAD51, the eukaryotic homologous recombinase. We identified the BRCA2 homolog in a Basidiomycota yeast,Naganishia liquefaciens. BRCA2 homologs are found in many Basidiomycota species but not in Ascomycota species.NaganishiaBRCA2 (Brh2, forBRCA2homolog) is about one-third the size of human BRCA2. Brh2 carries three potential BRC repeats with two oligonucleotide/oligosaccharide-binding domains. The homolog of DSS1, a small acidic protein serving as an essential partner of BRCA2 was also identified. The yeast two-hybrid assay shows the interaction of Brh2 with both Rad51 and Dss1. Unlike human BRCA2, Brh2 is not required for normal cell growth, whereas loss of Dss1 results in slow growth. The loss of Brh2 caused pronounced sensitivity to UV and ionizing radiation, and their HR ability, as assayed by gene-targeting efficiency, is compromised. These phenotypes are indistinguishable from those of therad51mutant, and therad51 brh2double mutant.NaganishiaBrh2 is likely the BRCA2 ortholog that functions as an indispensable auxiliary factor for Rad51.
Funder
MEXT | Japan Society for the Promotion of Science
Takeda Science Foundation
Publisher
Life Science Alliance, LLC
Subject
Health, Toxicology and Mutagenesis,Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Ecology