Horizontal transfer of therfbcluster inLeptospirais a genetic determinant of serovar identity

Author:

Nieves Cecilia1ORCID,Vincent Antony T12,Zarantonelli Leticia3ORCID,Picardeau Mathieu45,Veyrier Frédéric J1ORCID,Buschiazzo Alejandro35ORCID

Affiliation:

1. Bacterial Symbionts Evolution, Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique, Université du Québec, Laval, Canada

2. Département des Sciences Animales, Faculté des Sciences de l’agriculture et de l’alimentation, Université Laval, Quebec City, Canada

3. Laboratory of Molecular and Structural Microbiology, Institut Pasteur de Montevideo, Montevideo, Uruguay

4. Institut Pasteur, Université Paris Cité, CNRS UMR 6047, Biology of Spirochetes Unit, Paris, France

5. Integrative Microbiology of Zoonotic Agents, Pasteur International Joint Research Unit, Paris/Montevideo, France/Uruguay

Abstract

Leptospirabacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans. These spirochetes exhibit large intraspecific variation, resulting in complex tabulations of serogroups/serovars that crisscross the species classification. Serovar identity, linked to biological/clinical phenotypes, depends on the structure of surface-exposed LPS. Many LPS biosynthesis–encoding genes reside within the chromosomicrfbgene cluster. However, the genetic basis of intraspecies variability is not fully understood, constraining diagnostics/typing methods to cumbersome serologic procedures. We now show that the gene content of therfbcluster strongly correlates withLeptospiraserovar designation. Whole-genome sequencing of pathogenicL. noguchii, including strains of different serogroups, reveals that therfbcluster undergoes extensive horizontal gene transfer. Therfbclusters from severalLeptospiraspecies disclose a univocal correspondence between gene composition and serovar identity. This work paves the way to genetic typing ofLeptospiraserovars, and to pinpointing specific genes within the distinctrfbclusters, encoding host-specific virulence traits. Further research shall unveil the molecular mechanism ofrfbtransfer amongLeptospirastrains and species.

Funder

Institut Pasteur

Institut Pasteur & Institut Pasteur de Montevideo

Natural Sciences and Engineering Research Council of Canada

Publisher

Life Science Alliance, LLC

Subject

Health, Toxicology and Mutagenesis,Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Ecology

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