Migration of dermal cells expressing a macrophage C-type lectin during the sensitization phase of delayed-type hypersensitivity
Author:
Chun Kyung-hee1,
Imai Yasuyuki2,
Higashi Nobuaki1,
Irimura Tatsuro1
Affiliation:
1. Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
2. Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka , Japan
Abstract
Abstract
Dermal cells expressing a macrophage C-type lectin (mMGL) were previously suggested to migrate to regional lymph nodes during the sensitization phase of delayed-type hypersensitivity (DTH). The migration seemed to be induced by the solvent used to dissolve the antigen, and the DTH response was significantly enhanced by the migration. In this study, immunohistochemical analysis of skin after epicutaneous application of one of such solvents, a mixture of acetone and dibutylphthalate (AD), revealed a transient decrease in the number of mMGL-positive cells in the dermis. A similar decrease in this cell population was also observed in an ex vivo assay with skin explants excised from AD-treated sites. Conditioned medium from organ culture of AD-treated skin induced a similar decrease of mMGL-positive cells in untreated dermis, indicating the involvement of soluble factors. mMGL-positive cells seemed to represent a unique subpopulation of F4/80-positive dermal cells.
Funder
Ministry of Education, Science, Sports, and Culture of Japan
Research Association for Biotechnology
Promotion of Basic Research Activities for Innovative Biosciences
Cosmetology Research Foundation
Publisher
Oxford University Press (OUP)
Subject
Cell Biology,Immunology,Immunology and Allergy
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