Investigation of the Effect of Disinfectants on Biofilm Forming Non-Fermenting Bacteria

Abstract

The relevance of this study is determined by the wide prevalence in medical practice of biofilm infections caused, in particular, non-fermenting gram-negative bacteria with increased resistance to antibiotics and disinfectants. The aim of this study was to study the effect of disinfectants on biofilm formation of non-fermenting bacteria and to assess the possibility of using a mass spectrometric method to optimize the selection of disinfectants that affect the process of biofilm formation in non-fermenting bacteria. Results and conclusions. The drugs of choice for the reliable elimination of non-fermenting bacteria was hydro-gen peroxide and Ultradon, which microbially effect close to 100 %. The used solutions of disinfectants showed the same effect on the ability of non-fermenting bacteria to biofilm formation: the use of sublethal concentrations of these disinfectants led to a synchronous decrease in biofilm formation. The advantage of the mass spectrometric method of studying the sensitivity of non-fermenting bacteria to disinfectants is its objectivity, speed and ease of execution. The method gives an economic effect on time, labor and consumables compared to the traditionally used method of serial dilutions and the determination of viable clones of bacteria. Allows to investigate simultaneously sensitivity of cul-tures to different disinfectants on an assessment of denaturation of proteins with the subsequent change of mass spectrometric profiles.