COLLECTION OF THE STRAINS TESCHOVIRUS A, SAPELOVIRUS A, ENTEROVIRUS G OF THE INSTITUTE OF AGRICULTURAL MICROBIOLOGY AND AGROINDUSTRIAL MANUFACTURE OF THE NAAS

Abstract

Objective. Arrangement of the collection of porcine enteroviruses (PEV) strains isolated on the territory of Ukraine in accordance with the requirements of the International Committee on Virus Taxonomy and supplementing it with new strains Teschovirus A (TV-A), Sapelovirus A (SV-A), Enterovirus G (EV-G). Methods. Virological, serological, molecular genetic, instrumental and statistical. Isolation, cultivation of viruses and determination of their biological activity were performed in passaged culture of porcine embryonic kidney cells (СНЕВ). The viral titre was calculated by the method of Reed and Muench. The typical affiliation of viruses was determined in the virus neutralization reaction in СНЕВ cell culture. Species affiliation was determined by reverse transcription polymerase chain reaction (RT PCR) using species-specific primers for TV-A, SV-A and EV-G, developed by us. Electron microscopy of viruses was performed on a transmission electron microscope by negative contrast enhancement method. Statistical processing was performed in Microsoft Office Excel and StatSoft STATISTICA 12. Results. As a result of the epizootic survey during 2002– 2019, 1,216 samples for virological testing were selected. Successive passages of СНЕВ cell culture resulted in obtaining 274 viral isolates. According to the results of studying physicochemical, morphological, biological properties of these isolated, they are classified as PEV. In connection with the change of taxonomy and nomenclature of PEV, serological and genetic reclassification of 30 strains of viruses isolated in Ukraine, including 14 reference strains according to the classification of V. P. Romanenko, 7 production strains, 9 strains with polyantigenic properties and 4 strains that did not have antigenic affinity with viruses of known PEV serotypes according to the classification of V. P. Romanenko was performed. It has been established that the reference strains of PEV according to the trivial classification of V. P. Romanenko belong to the species TV-A of the genus Teschovirus. As a result of conducted serological testing, PEV-10 M 2323, PEV-12 K 22, PEV-13 L 90, PEV-14 M 116, PEV-16 G 95, PEV-17 V 111, PEV-18 Ch 184, PEV-19 D 227, PEV-20 I 249, PEV-23 I 393 were classified as TV-A1; PEV-11 K 9, PEV-15 Ch 73 — as TV-A3, PEV-22 V 151 — as TV-A6. PEV-21 P 142 did not have antigen affinity with reference strains TV-A, SV-A and EV-G and belongs to a new serotype. Production strains of PEV-1 Perechynskyi 642, Bereznianskyi 652, Chernihivskyi 2372 were reclassified as TV-A1. PEV strains with polyantigenic properties such as G 31 and L 2661 have intertypic antigens with TV-A 1, 10, 11 and TV-A 3, 6, 10 serotypes, respectively. PEV strain of a new serotype Ch 881 was reclassified as SV-A. PEV strains Т 3, Ch 863, Ch 878 are the new serotypes of TV-A. Conclusion. As a result of studies, 274 viral isolates were isolated from 1,216 samples of material. The collection was supplemented with 20 reference strains of Teschovirus A, Sapelovirus A and Enterovirus G. Genetic and serological reclassification of 30 PEV strains isolated in Ukraine was performed. The collection of viral strains has been arranged in accordance with modern taxonomy and nomenclature. Seven viral strains were deposited. The collection of viruses has been supplemented with 4 strains of new serotypes of Teschovirus A.