New Insights into Coenzyme a Interaction with Mercury Ions Provided by Mass Spectrometric and Circular Dichroism Spectroscopic Approaches-Reference-Cited by-同舟云学术

New Insights into Coenzyme a Interaction with Mercury Ions Provided by Mass Spectrometric and Circular Dichroism Spectroscopic Approaches

Published:2015-04 Issue:2 Volume:21 Page:97-102
ISSN:1469-0667
Container-title:European Journal of Mass Spectrometry
language:en
Short-container-title:Eur J Mass Spectrom (Chichester)

Author:

Andries Claudia¹,Manea Marilena²,Drochioiu Gabi¹,Gradinaru Robert¹

Affiliation:

1. Alexandru Ioan Cuza University of Iasi, Department of Chemistry, 11 Carol I, Iasi 700506, Romania

2. University of Konstanz, Department of Chemistry and Zukunftskolleg, 78457 Konstanz, Germany

Abstract

The interaction of coenzyme A (CoA) with mercury ions was investigated using electrospray ionization mass spectrometry and circular dichroism spectroscopy. Our results indicated a 1:1 stoichiometric CoA–Hg complex at physiological pH. Furthermore, the CoA conformation considerably changed in the presence of mercury ions. In addition, a by-product of the reaction, thiocoenzyme A, was identified using mass spectrometry.

Publisher

SAGE Publications

Subject

Spectroscopy,Atomic and Molecular Physics, and Optics,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1255/ejms.1361

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