Time-of-Flights and Traps: From the Histone Code to Mars

Author:

Cotter Robert J.1,Swatkoski Stephen1,Becker Luann2,Evans-Nguyen Theresa1

Affiliation:

1. Middle Atlantic Mass Spectrometry Laboratory, Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

2. Physics and Astronomy, College of Arts and Sciences, Johns Hopkins University, Baltimore, MD 21218, USA

Abstract

Two very different analytical instruments are featured in this perspective paper on mass spectrometer design and development. The first instrument, based upon the curved-field reflectron developed in the Johns Hopkins Middle Atlantic Mass Spectrometry Laboratory, is a tandem time-of-flight mass spectrometer whose performance and practicality are illustrated by applications to a series of research projects addressing the acetylation, deacetylation and ADP-ribosylation of histone proteins. The chemical derivatization of lysine-rich, hyperacetylated histones as their deuteroacetylated analogs enables one to obtain an accurate quantitative assessment of the extent of acetylation at each site. Chemical acetylation of histone mixtures is also used to determine the lysine targets of sirtuins, an important class of histone deacetylases (HDACs), by replacing the deacetylated residues with biotin. Histone deacetylation by sirtuins requires the co-factor NAD+, as does the attachment of ADP-ribose. The second instrument, a low voltage and low power ion trap mass spectrometer known as the Mars Organic Mass Analyzer (MOMA), is a prototype for an instrument expected to be launched in 2018. Like the tandem mass spectrometer, it is also expected to have applicability to environmental and biological analyses and, ultimately, to clinical care.

Publisher

SAGE Publications

Subject

Spectroscopy,Atomic and Molecular Physics, and Optics,General Medicine

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Robert J. Cotter 1943–2012/Frank H. Field 1922–2013;Journal of the American Society for Mass Spectrometry;2024-08-07

2. Unveiling the Intricacies of the Curved-Field Ion Mirror;European Journal of Mass Spectrometry;2015-06

3. Current literature in mass spectrometry;Journal of Mass Spectrometry;2010-10

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