Electrospray Ionization–Collision-Induced Dissociation–Tandem Mass Spectrometry Study of Lead Complexes with Deprotonated Nucleobases

Abstract

The complexes between the lead cation and deprotonated nucleobases (and deprotonated nucleosides) are studied by using electro–spray ionization–collision-induced dissociation–tandem mass spectrometry. It has been found that the deprotonated N9 atom is not the site of lead cation attachment. In ions [A – H + Pb]+ and [C – H + Pb]+, the lead cation is coordinated by the adenine N1 atom and cytosine N3 atom and interaction between the lead cation and deprotonated amino groups seems very likely. Deprotonated thymine shows a higher affinity toward lead cation than deprotonated uracil. In the lead–nucleoside complexes lead cation interacts with a sugar moiety.