Dnmt3a and Dnmt3b expressions decrease after cryopreservation and re-transplantation of ovarian tissue-Reference-Cited by-同舟云学术

Dnmt3a and Dnmt3b expressions decrease after cryopreservation and re-transplantation of ovarian tissue

Published:2023-01-01 Issue:1 Volume:9 Page:82-89
ISSN:2687-2781
Container-title:Akdeniz Medical Journal
language:tr
Short-container-title:Akd Tıp D

Author:

UYSAL Fatma¹,ÇELİK Soner²,TOPAL CELİKKAN Ferda³,ÇELİK-ÖZENCİ Çiler²,ÖZKAVUKÇU Sinan³,CAN Alp³,AKKOYUNLU Gökhan²

Affiliation:

1. ANKARA MEDİPOL ÜNİVERSİTESİ, TIP FAKÜLTESİ

2. AKDENİZ ÜNİVERSİTESİ, TIP FAKÜLTESİ

3. ANKARA ÜNİVERSİTESİ, TIP FAKÜLTESİ

Abstract

Objective DNA methylation includes maintenance and de novo methylation process that are catalyzed by DNA methyltransferase (Dnmt) enzymes and these enzymes possess crucial roles in methylation of genes associated with the development of ovarian follicles. The aim of this study is to evaluate whether expression of Dnmt3a and Dnmt3b protein levels change after cryopreservation and re-transplantation of ovarian tissue in rats. Methods Four groups were designed as; fresh control (FC), frozen/thawed (FT), fresh re-transplanted (T), and frozen/thawed and re-transplanted (FTT). Slow freezing was used to cryopreserve the ovarian tissue pieces and expression of Dnmt3a and Dnmt3b proteins was assessed by immunohistochemistry. Result Expression of Dnmt3a and Dnmt3b significantly decreased in ovarian follicles in FT, T and FTT groups when compared FC. Conclusion Expression of Dnmt enzymes has been altered both after cryopreservation alone and following re-transplantation cycles which may lead to disturbed DNA methylation processes during rat folliculogenesis

Publisher

Akdeniz University

Reference30 articles.

1. 1. Huffman, S.R., Y. Pak, and R.M. Rivera, Superovulation induces alterations in the epigenome of zygotes, and results in differences in gene expression at the blastocyst stage in mice. Mol Reprod Dev, 2015. 82(3): p. 207-17.

2. 2. Hu, W., et al., Effect of slow freeze versus vitrification on the oocyte: an animal model. Fertil Steril, 2012. 98(3): p. 752-760 e3.

3. 3. Zhao, X.M., et al., Effect of vitrification on promoter CpG island methylation patterns and expression levels of DNA methyltransferase 1o, histone acetyltransferase 1, and deacetylase 1 in metaphase II mouse oocytes. Fertil Steril, 2013. 100(1): p. 256-61.

4. 4. Halliday, J., et al., Beckwith-Wiedemann syndrome and IVF: a case-control study. Am J Hum Genet, 2004. 75(3): p. 526-8.

5. 5. Hansen, M., et al., The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization. New England Journal of Medicine, 2002. 346(10): p. 725-730.