The effect of plant growth regulator bacteria on micro propagation of grapevine rootstock with three different rooting abilities

Abstract

In the study, 3 American rootstocks (110 R, 1103 P and 5 BB) with low, medium, and high rooting ability were used, 2 different bacterial isolates Bacillus cereus (ZE-7) and Pseudomonas putida (ZE-12) and their binary combination and the bottom of the cuttings. It was aimed to determine the effect of bacteria on rooting by treating the rooting part. During the rooting stage, hormone-free MS medium was treated with PGPRs, and in addition, hormone-free MS medium containing 1 mgl-1 IBA was used to determine the effects of PGPRs strains. At the end of the study, the root ratio of the explants was maintained; root length, number of roots, root fresh weight, root dry weight, shoot length, shoot fresh weight, and shoot dry weight data were examined. It was determined that PGPR applications generally gave higher values than control and IBA applications. Among the rootstocks, it was determined that they gave the best results in terms of root development. The highest values were obtained in the 5BB rootstock, and the lowest values were generally obtained in the 110R rootstock. Shoot development values varied according to the rootstocks and applications. In terms of the effects of the applications on root development, the highest rooting rate was 72.03%, the highest root number ratio was 1.95 on average, and the highest root fresh weight was 39.75 mg from the Bacillus Cereus application; Additionally, the highest root dry weight was obtained from B.cereus+P.putida application with 13.06 mg. The research highlighted this feature of 5 BB rootstock, which is considered among the easily rooting rootstocks, with its PGPR effect. 110 R, which is known as a difficult rooting rootstock and has high resistance to drought and active lime, which are the biggest threats today, showed a lower rooting rate, but when the values were examined, it was above the control and IBA applications.