Non-specific activity of Staphylococcus aureus strains producing and not producing cytokine-like substances assessed by immuno-enzyme analysis

Abstract

A cytokine profile covering cytokine level in various biological fluids isa of great importance un ummunbe-based diagnostics, examinign parameters and stated bof functional asctivity of immune system organs. While using ELISA asw oner of the most common diagnostics tools, it is worth taking into account origin of biological samples. Daily Staphylococcus aureus broth culture supernaturants, positive and negative for cytokine-like substances, were used to determine non-specific activity by using enzyme immunoassay using kits pruchased from the JSC “Vector-Best” (Novosibirsk) as well as kits of LLC “HEMA” (Moscow) to diagnose some infections and hormone levels. We found that in supernatants from some Staphylococcus aureus strain producing cytokine-like substances identical to human, the absence of non-specific cross-activity assessed by ELISA was documednted exсept for the HBsAg test kit, which may indicate high specificity of the other kits studied and indirectly confirm an assumption that staphylococci secrete proteins specifically reacting mainly with antibodies against human cytokines, but can also cross-react with other antigens, but not antibodies.