Evaluation of triterpenoid miliacin-related anti-mutagenic effect in mice exposed to ionising radiation
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Published:2020-04-15
Issue:2
Volume:23
Page:153-156
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ISSN:1028-7221
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Container-title:Russian Journal of Immunology
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language:
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Short-container-title:Russian Journal of Immunology
Author:
Sarycheva Yu. A.,Tokareva A. A.,Shtil A. A.,Kolyvanova M. A.,Morozov V. N.,Panfilova T. V.,Frolov B. A.
Abstract
Triterpenoid miliacin-related anti-mutagenic effect aftert exposure to isonizing radiation was studied to assess its protective properties against radiation-induced immunosuppression. (CBAxC57Bl6)F1 mice were subdivided into four groups: 1) intact mice; 2) irradiated mice; 3) miliacin-pretreated irradiated mice; and 4) miliacin-solvent-pretreated irradiated mice. Irradiation of animals was performed on the X-ray device “RUST-M1” (4 Gy; exposure time 288 sec.). Twenty four hours post irradiation myelokaryocytes were isolated for analysis. Chromosome preparations were examined by light microscopy. Injections of miliacin (4 mg/kg daily for 3 consecutive days) attenuated the irradiation-triggered mutagenic effect assessed by counting cells with chromosomal aberrations as well as number of aberrations per 100 metaphase plates. Miliacin exerted a protective effect on radiation-induced chromosomal aberrations, although degree of protection was less pronounced compared to chemically induced mutagenesis. These data indicate about limited potential for miliacin to protect central immune organs such as red bone marrow upon radiation exposure.
Publisher
Russian Society of Immunology
Subject
Immunology,General Medicine
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