State of hyperglycemic and immunomodulator-treated peripheral blood lymphocytes-Reference-Cited by-同舟云学术

State of hyperglycemic and immunomodulator-treated peripheral blood lymphocytes

Published:2020-07-15 Issue:3 Volume:23 Page:329-334
ISSN:1028-7221
Container-title:Russian Journal of Immunology
language:
Short-container-title:Russian Journal of Immunology

Author:

Gette I. F.,Danilova I. G.

Abstract

A level of peripheral blood lymphocytes, their functional activity and particularly produced range of secreted cytokines underlie severity of autoimmune process in diabetes mellitus (DM) against islet β-cells and other body cells. Because cytokine production is coordinated by macrophages and may also depend on accessibility lymphocyte nucleic acids, we aimed at examining level of nucleic acids in blood lymphocytes as well as cytokine production in rats with alloxan-induced diabetes treated with immunomodulatory 3-aminophthalhydrazide (3-APH). Type 1 DM was modeled in male WISTAR rats by intraperitoneally administered alloxan at a dose of 300 mg/kg followed by inoculating 3-APH (2 mg/kg, in total 20 intramuscular injections). It was found that modeled diabetes was accompanied by increased number of peripheral blood lymphocytes and their bone marrow precursors, coupled to higher DNA amount in blood lymphocytes and associated with increased IL-6 and TNFα production. Administration of 3-APH to diabetic rats contributed to decreased number of peripheral blood lymphocytes and related DNA level likely resulting in decreased level of pro-inflammatory cytokine IL-6 in the blood serum and contributing to corrected hyperglycemia.

Publisher

Russian Society of Immunology

Subject

Immunology,General Medicine

Reference8 articles.

1. Гетте И.Ф. Содержание гистоновых белков в лимфоцитах крыс с аллоксановым диабетом в условиях модулирования активности макрофагов // Вестник уральской медицинской академической науки, 2014, № 3 (49). С. 18-19. [Gette I.F. Content of histone proteins in lymphocytes of rats with alloxan diabetes in terms of macrophage activity modulating. Vestnik uralskoy meditsinskoy akademicheskoy nauki = Bulletin of the Ural Medical Academic Science, 2014, no. 3 (49), pp. 18-19. (In Russ.)]

2. Ланкин В.З., Тихазе А.К., Коновалова Г.Г., Одинокова О.А., Дорощук Н.А., Чазова И.Е. Оксидативный и карбонильный стресс как факторы модификации белков и деструкции ДНК при сахарном диабете // Терапевтический архив, 2018, № 10. С. 46-50. [Lankin V.Z., Tikhaze A.K., Konovalova G.G., Odinokova O.A., Doroshchuk N.A., Chazova I.E. Oxidative and carbonyl stress as a factors of the modification of proteins and DNA destruction in diabetes. Terapevticheskiy arkhiv = Therapeutic Archive, 2018, Vol. 90, no. 10, pp. 46-50. (In Russ.)]

3. Маркушева Л.И., Савина М.И., Решина В.М., Тогузов Р.Т. Ядерные белки хроматина в оценке эффективности лечения больных псориазом // Клиническая лабораторная диагностика, 2000. № 7. С. 18-20. [Markusheva L.I., Savina M.I., Reshina V.M., Toguzov R.T. Chromatin nuclear proteins in evaluation of the effectiveness of treatment of patients with psoriasis. Klinicheskaya laboratornaya diagnostika = Russian Clinical Laboratory Diagnostics, 2000, no. 7, pp. 18-20. (In Russ.)]

4. Сычева Л.П. Биологическое значение, критерии определения и пределы варьирования полного спектра кариологических показателей при оценке цитогенетического статуса человека // Медицинская генетика, 2007. № 11. С. 3-11. [Sycheva L.P. Biological value, scoring criteria and limits of a variation of a full spectrum karyological indexes of exfoliated cells for estimation of human cytogenetic status. Meditsinskaya genetica = Scientific and Practical Journal Medical Genetics, 2007, no. 11, pp. 3-11. (In Russ.)]

5. Danilova I.G., Bulavintceva T.S., Gette I.F., Medvedeva S.Y., Emelyanov V.V., Abidov M.T. Partial recovery from alloxan-induced diabetes by sodium phthalhydrazide in rats. Biomed. Pharmacother., 2017,Vol. 95, pp. 103-110.