Rapid and efficient method to make competent bacterial cells (Preprint)

Abstract

Genetic transformation involves the alteration of genes when cells take up uncoded Deoxyribonucleic acid from the extracellular environment.

To render competency to untransformeed bacterial cells using the Calcium chloride cation.

The heat-shock method perforates the cell membrane to make it permeable for the foreign DNA to penetrate easily.

Viable colonies will take up the gene construct, cloned into a plasmid, thus making the bacterial cells capable of replicating the desired sequence, in sufficient numbers

Competent bacterial cells can be used in diverse molecular analyses, such as plant genetic transvia Agrobacterium mediated transgenic system.