New Smartphone Based Colorimetric Method Development and Validation of Emtricitabine in Bulk and Tablet Dosage Form
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Published:2021-07-15
Issue:4
Volume:11
Page:35-40
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ISSN:2250-1177
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Container-title:Journal of Drug Delivery and Therapeutics
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language:
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Short-container-title:J. Drug Delivery Ther.
Author:
Mane Yashashree,Mashru Rajashree
Abstract
The novel smartphone based colorimetric application called PhotoMetrix-PRO has been used for quantitative analysis of Emtricitabine in bulk and tablet dosage form and it compared with conventional UV –visible spectrophtotometry. Smartphone based colorimetric method and UV method was based on detection of intensity of color with increasing concentration Ammonium metavanadate (inorganic oxidizing agent) which was used for preparation Mandeline reagent they used as coloring agent for colour development .The reagent is orange red colour but when it react with Emtricitabine it convert into green color complex. The developed methods have good linearity 50-250μg/ml. The color intensity of API increase with increasing concentration. All images were captured through mobile phone and analysed in PhotoMetrix PRO application. This application convert image into Red, Green and Blue(RGB)histogram and regression models were built into PhotoMetrix-PRO Application both the method have correlation coefficient R2>0.995.The LOD and LOQ for PhotoMetrix PRO 4.77μg/ml and14.92μg/ml and UV-visible spectrophotometry method 4.99 μg/ml and 14.96 μg/ml respectively. The % RSD of Emtricitabine by PhotoMetrix PRO and UV method was 99% (%RSD <2). Appling statistical tool i.e paired two test on both the methods result indicate that both methods are equally significant.
Keywords: Smartphone, PhotoMetrix PRO, UV Spectrophotometry, RGB
Publisher
Society of Pharmaceutical Tecnocrats
Cited by
1 articles.
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