FORMULATION AND CHARACTERIZATION OF FACIAL SERUM FROM ASTAXANTHIN-BETA CAROTENE NANOEMULSION AS AN ANTIOXIDANT

Abstract

Objective: The purpose of this study is to make facial serum from astaxanthin-beta carotene nanoemulsion as an antioxidant. Methods: Nanoemulsions were prepared using Spontaneous Nanoemulsion (SNE) method with a ratio of 1:8:1 for (Sunflower oil): surfactant (Kolliphor® RH40): and co-surfactant (PEG 400). Physical nanoemulsion characterization includes globule size, polydispersity index, zeta potential, visual appearance, pH and entrapment efficiency test. The best results from nanoemulsion were then combined into serum preparations which were then tested for evaluation of the preparations, including organoleptic, homogeneity, viscosity, pH, adhesion and stability test (cycling test). Results: The results showed that the nanoemulsion of astaxanthin and beta-carotene combination that had been developed had a globule size of<50 nm (with a normal globule size distribution curve), polydispersity index value was less than 0.5, zeta potential was greater than-20 mV and entrapment efficiency was ranging from 80-85%. The results of the preparation evaluation showed that serum astaxanthin-beta carotene nanoemulsion had good results in physical, chemical and stability test during storage. The best serum formula is formula 3 when viewed from the results of the evaluation of the dosage form with an IC50 value of 8.562 ppm with a very strong category as an antioxidant. Conclusion: Facial serum from astaxanthin-beta carotene is the potential to be an antioxidant.