Abstract
Objective: The study aimed to estimate the prevalence of methicillin resistance of Staphylococcus aureus in various clinical samples received at tertiary care hospital. Initially, the Staphylococcus aureus and its antibiotic susceptibility tests is performed in clinical samples which are submitted to the department of Microbiology. And Methicillin Resistance Staphylococcus Aureus (MRSA) is determined by using cefoxitin (30 μg) as per CLSI guidelines.
Methods: The prospective study was conducted in department of Microbiology in a tertiary care hospital. All Staphylococcus aureus organisms isolated in clinical samples were included in the study and processed as per the standard operating procedure. Methicillin susceptibility was tested by using cefoxitin (30μg) disks on Muller-Hinton agar plates that were inoculated with a suspension (equal to 0.5 McFarland standards) of the s. aureus.
Results: In our study, amongst hundred staphylococcus aureus isolates, sixty isolates were shown resistance to cefoxitin (30µg), which indicates that percentage of methicillin-resistant s. aureus in our study is 60. Majority of s. aureus were isolated from blood samples 44% (n= 44) followed by pus samples 32% (n=32). Linezolid resistance reported was 3%. All isolates were sensitive to vancomycin and daptomycin by disc diffusion test as per CLSI guidelines 2021.
Conclusion: To conclude, MRSA plays a significant role and it can be transmitted through endogenous, cross-infection and reinfections. Phenotypic methods like use of cefoxitin disc (30µg) can be considered for detection of methicillin resistance in S. aureus, as it consumes less time and easy to perform.
Publisher
Innovare Academic Sciences Pvt Ltd
Reference22 articles.
1. Colley EW, McNicol MW, Bracken PM. Methicillin-resistant Staphylococci in a general hospital. Lancet. 1965;1(7385):595-7. doi: 10.1016/s0140-6736(65)91165-7, PMID 14250094.
2. Scragg JN, Appelbaum PC, Govender DA. The spectrum of infection and sensitivity of organisms isolated from African and Indian children in a Durban hospital. Trans R Soc Trop Med Hyg. 1978;72(4):325-8. doi: 10.1016/0035-9203(78)90118-9, PMID 705838.
3. Geyid A, Lemeneh Y. The incidence of methicillin resistant S. aureus strains in clinical specimens in relation to their beta-lactamase producing and multiple-drug resistance properties in Addis Abeba. Ethiop Med J. 1991;29(4):149-61. PMID 1954949.
4. Foster TJ. The staphylococcus aureus “superbug”. J Clin Invest. 2004;114(12):1693-6. doi: 10.1172/JCI23825, PMID 15599392.
5. Gupta K, Martinello RA, Young M, Strymish J, Cho K, Lawler E. MRSA nasal carriage patterns and the subsequent risk of conversion between patterns, infection, and death. Plos One. 2013;8(1):e53674. doi: 10.1371/journal.pone.0053674, PMID 23326483.