DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF SITAGLIPTIN AND METFORMIN IN BULK AND COMBINED PHARMACEUTICAL FORMULATION

Abstract

Objective: To develop simple, accurate, precise UV Spectrophotometric method for the simultaneous estimation of Sitagliptin and Metformin in tablet dosage form. Methodols: The method is based on the determination of Sitagliptin and Metformin in tablet using simultaneous equation method. Sitagliptin exhibits maximum absorbance at 267 and Metformin exhibits maximum absorbance at 237 nm using distilled water as diluents. Results: The calibration curve was linear in the range of 10-300 µg/ml for Sitagliptin and 4-14µg/ml for Metformin. The %RSD were within the limit i.e., less than 2%. The % recovery of the proposed method was found to be 97.12-99.46% for Sitagliptin and 98.15-99.85% for Metformin. The LOD of the proposed method was 0.397μg/ml for Sitagliptin and 0.8952µg/ml for Metformin. The LOQ was 1.2951μg/ml for Sitagliptin and 2.7159μg/ml for Metformin. Conclusion: A simple, accurate, precise UV Spectrophotometric method for the simultaneous estimation of Sitagliptin and Metformin in tablet dosage form.