COMPARATIVE MODELING FOR SACCHAROMYCES CEREVISIAE RAD56 USING SWISS-MODEL, I-TASSER, AND PHYRE2

Abstract

Objective: RAD56 is a protein its causes pathological conditions of Kohler disease, Mueller-Weiss syndrome, which leads to hindfoot pain. RAD56 is considered an impressive drug target for various illnesses. The experimental 3D structure of RAD56 is not available. Therefore, the present study aims in developing a homology model using 3 different software and evaluate the best model. Methods: The developing homology modeling on RAD56 is built utilizing three diverse software’s to be specific Swiss-Model, I-Tasser, and Phyre2. All the predicted models were analyzed and approved by PROCHECK, PROSA, Errat, and Verify_3D. Results: Homology Modeling anticipated from Swiss-Model appeared best comes about with 88.6% of the buildups within the most favorable locale, 11.2% within the permitted region, 0.6% within the liberally permitted locale, and 0.2% within the refused locale. PROCHECK, PROSA, Errat, and Verify_3D, too, affirmed the same. Conclusion: Homology Modeling was created for RAD56 utilizing Swiss-Model, I-Tasser, and Phyre2. The models created were validated utilizing PROCHECK, PROSA, Errat, and Verify_3D. This investigation approved the homology model created by is best Swiss-Model 88.6, vigorous as well as solid sufficient to be utilized for future pondering.