INVESTIGATION OF ANTIBACTERIAL ACTIVITY OF METHANOLIC EXTRACT AND ISOLATION OF STEROL AND TRITERPENOIDS FROM GREWIA TILIAEFOLIA VAHL LEAF

Abstract

Objective: In the present study, we have discussed the antibacterial activity of methanolic extract of Grewia tiliaefolia Vahl leaf and the isolation of pure phytochemicals by using column chromatography. Methods: The powdered material was extracted with methanol using a soxhlet extractor and isolation of compounds by column chromatography. Evaluation of antibacterial activity determined the zone of inhibition by disc diffusion method. Results: Three compounds were isolated from methanolic extract of the leaf of Grewia tiliaefolia using column chromatography, namely daucosterol, lupeol, and friedelin were purified after isolation and characterized by using UV, FTIR, NMR, MASS spectroscopic techniques, and antimicrobial activity was carried out on the crude methanolic extract a higher zone of inhibition was observed against Staphylococcus aureus with a zone diameter of 34.1±0.513 mm at a concentration of 10 mg/ml. Conclusion: The results observed in this research work conclude that the methanolic extract showed good antibacterial activity may be due to the compounds isolated like daucosterol, lupeol, and friedelin.