ANTIMICROBIAL AND PHYTOCHEMICAL INVESTIGATION OF CALOTOROPIS PROCERA FLOWERS EXTRACTS

Author:

ALTAYEB SARA HUSSEINORCID,KHALID ASAAD

Abstract

Objective: The present study was planned to screen extracts of different polarities of the flowers of Calotropis procera for the detection of different secondary metabolites, estimate the antibacterial activity of the prepared extracts, and study the active extracts by different chromatographic and spectroscopic methods. Methods: The diethyl ether, methanol, and water extracts were phytochemically screened. Petroleum ether, chloroform, and methanol extracts were also tested against two Gram-positive bacteria, namely Bacillus subtilis and Staphylococcus aureus, and two Gram-negative bacteria, namely E. coli and Pseudomonas aeruginosa with diffusion method. The methanolic extract was further investigated by column chromatography (CC) and preparative thin-layer chromatography (PTLC). Three pure compounds have been isolated and investigated by IR-spectroscopy. Results: Phytochemical screen showed the presence of various secondary metabolites such as flavonoids, alkaloids, steroids, cardiac glycosides, reducing sugars, and saponins. The antibacterial assay revealed that the methanolic extract was the most active against the tested bacteria, especially against Pseudomonas aeruginosa with the high zone of inhibition (23 mm) at 100 mg/ml, and E. coli (22 mm) at 100 mg/ml, followed by chloroform extract, while the petroleum ether extract was insignificantly active. Column Chromatography analysis of the methanolic extract separated fifteen fractions. The PTLC of fraction No.14 enabled the isolation of three pure compounds (A, B, and C). The IR-spectroscopy analysis of the three isolated compounds exhibited that they could referred to the alkaloids or cardiac glycosides. Conclusion: The methanolic extract showed significant activity against tested bacteria, especially E. coli and Pseudomonas aeruginosa. The result also indicates the presence of secondary metabolites in C. procera extracts. Subsequently the therapeutic efficacy compounds isolated and purified from C. procera could be used as an important source against bacterial ailments in humans and plants.

Publisher

Innovare Academic Sciences Pvt Ltd

Subject

Pharmaceutical Science,Pharmacology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3