EVALUATION OF IN VIVO WOUND HEALING ACTIVITY OF SOLANUM VIRGINIANUM ROOT EXTRACT ON EXCISION AND INCISION WOUND MODEL IN RATS

Abstract

Objective: Evaluation of the wound healing activity of Solanum virginianum root extracts using excision and incision wound models.  Methods: Moisture content, Ash value, Acid insoluble ash value, Water-soluble ash value, and different extractive values were studied as physicochemical parameters. Qualitative screening of each extract was carried out to determine different types of phytochemicals. In addition, a pharmacological study was conducted on groups of rats to find the effectiveness of root extracts (aqueous and alcoholic) on the wound healing process.  Results: Following observations were noted after the physicochemical test: Moisture content, 3.2%; Ash value, 5.77%; Acid insoluble ash, 1.17%; Water-soluble ash, 7.27%; Alcohol soluble extractive, 16.77%; Water-soluble extractive, 17.02%. After a qualitative chemical examination of alcohol and aqueous root extract, alkaloids, carbohydrates, phytosterols, saponins, phenolics, proteins, and flavonoids were detected. The excision wound model showed wound contraction for the alcohol extract group on the 12th day at 90.78% and the aqueous group at 85.23%. In the incision wound model, the alcohol extract-treated group showed significant wound breaking strength compared to the control and the aqueous extract-treated group. Conclusion: This study on the root was carried out to find evidence of the wound healing activity of its extracts. Results indicate the effectiveness of the extracts in enhancing the wound healing process in rat models. The result of qualitative screening enhances the future scope for various pharmacological approaches.