Abstract
Objective: The purpose of this study was to study the bacteriological profile of UTI in patients attending the tertiary care hospital and to study the antimicrobial sensitivity pattern of uropathogens.
Methods: This cross-sectional study was conducted after obtaining clearance from the institutional ethics committee. Clean-catch mid-stream urine samples were collected from patients symptomatic of UTIs. Samples were cultured aerobically on CLED agar. Isolates having significant growth (>105CFU/ml) were further processed for identification using standard microbiological techniques and their antimicrobial susceptibility pattern was evaluated by the Standard Kirby Bauer disk diffusion method as per CLSI 2020 guidelines.
Results: A total of 480 urine samples were processed, yielding 174 isolates. Escherichia coli (42.50%) was predominant, followed by Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter spp., Proteus spp., Providencia spp., Enterococcus spp., Citrobacter spp. and Morganella morganii. Gram-positive isolates exhibited high sensitivity towards vancomycin, linezolid, meropenem, and piperacillin tazobactum. Enteric coliforms exhibited high sensitivity towards colistin, meropenem, aminoglycosides, and piperacillin tazobactum. Non-fermenters exhibited high sensitivity towards colistin, meropenem, cefepime, and amoxycillin clavulanate.
Conclusion: The rampant injudicious irrational overuse of antibiotics has led to the emergence of multi-drug resistant bugs, which is posing a serious challenge to clinicians in the management of infections. Developing therapeutic protocols guided by susceptibility profiles for tuning antibiotic therapy regimens is an important strategy in tackling this menace.
Publisher
Innovare Academic Sciences Pvt Ltd
Subject
Pharmaceutical Science,Pharmacology
Reference39 articles.
1. Ronald AR, Pattullo AL. The natural history of urinary infection in adults. Med Clin North Am. 1991;75(2):299-312. doi: 10.1016/s0025-7125(16)30455-2, PMID 1996035.
2. Bano S, Tunio SA, Menon AA, Detho H, Bano R, Kumari K. Evaluation of antibiotic susceptibility pattern of uropathogens circulating in Hyderabad, Pakistan. Khyber Med Univ J. 2014;6(3):110-5.
3. Sohail M, Khurshid M, Murtaza Saleem HG, Javed H, Khan AA. Characteristics and antibiotic resistance of urinary tract pathogens isolated from Punjab, Pakistan. Jundishapur J Microbiol. 2015;8(7):1-4. doi: 10.5812/jjm.19272v2.
4. Patel S, Taviad PP, Sinha M, Javadekar TB, Chaudhari VP. Urinary tract infections (UTI) among patients at G.G. Hospital and Medical College, Jamnagar. Nat J Community Med. 2012;3(1):138-41.
5. Collee JG, Marr W. Laboratory strategy in the diagnosis of infective syndromes. In: Collee JG, Duguid JP, Fraser AG, Marmion BP, Simmons A, editors. Mackie and McCartney practical medical microbiology. 14th ed. New York: Churchill Livingstone; 2006. p. 53-94.