MORTALITY PREVENTION IN SEPTIC MICE WITH MULTIPLE ORGAN DYSFUNCTION SYNDROME: THE OPTIMAL DOSE OF INTRAPERITONEAL HEAT SHOCK PROTEIN 70

Abstract

Objective: Heat shock protein 70 (HSP70) reduces the expression of cytochrome C, B-cell lymphoma-associated X, and cysteine-aspartic acid protease 3 (Caspase 3), in apoptosis of multiple organ dysfunction syndrome (MODS), and thereby can prevent death. Previous researchers used 226 mg/kgBW/intraperitoneal (ip) of HSP70. This study aimed to determine the lowest effective dose of HSP70 to prevent death in sepsis mice model with MODS.Methods: This was a randomized control trial conducted at Pusat Antar Universitas (PAU), Gadjah Mada University, Yogyakarta, Indonesia, from April 1, 2017 to April 21, 2017. The study subjects were Balb/c strain mice. The drug used to induce death was lipopolysaccharides (LPS). The drug used to prevent death in this study was Rat HSP70. A sample of 25 mice was randomized into five groups with each consisting of five mice: (1) Control group with NaCl, (2) experimental group receiving LPS injection of 0.25 mg/kgBW/i.p, (3) experimental group receiving the same dose of LPS injection with HSP70 injection of 100 μg/kgBW/ip, (4) experimental group receiving the same dose of LPS injection with HSP70 injection of 200 μg/kgBW/ip, and (5) experimental group receiving the same dose of LPS injection with HSP70 injection of 300 μg/kgBW/ip. Percentage of live mice between groups was compared by Chi-square test.Results: 3 days after intervention, 13 (86.7%) live mice in the experimental group with ≥100 μg/kgBW/i.p HSP70 were >2 (40%) live mice in the experimental group with <100 μg/kgBW/i.p HSP70, with p=0.038. All mice receiving ≥200 μg/kgBW/ip HSP70 in the experimental group were Alive 3 days after intervention.Conclusion: The lowest effective dose of HSP70 to prevent death in sepsis mice model with MODS is 200 μg/kgBW/ip. All mice are alive 3 days after receiving ≥200 μg/kgBW/ip HSP70.