CYTOTOXIC EFFECT OF SYNTHESIZED SILVER NANOPARTICLES BY PULSE LASER ABLATION ON BREAST CANCER CELL LINE (AMJ13) FROM IRAQI PATIENT AND NORMAL HUMAN LYMPHOCYTES

Abstract

 Objective: Synthesized silver nanoparticles (AgNPs) in liquids were investigated as anticancer cells in the present study. Cytotoxic activities of six different concentrations 0.78, 1.56, 3.125, 6.25, 12.5, and 25 μg/ml of AgNPs against human breast cancer cell line (AMJ13) and lymphocytes were assessed with MTT assay.Methods: A Q-Switched Nd: YAG pulsed laser (λ=1064 nm, 800 mJ/pulse) was used for ablation of a pure silver plate to synthesis AgNPs in the polyvinylpyrrolidone and deionize distilled water. Ultraviolet-visible spectroscopy confirmed the synthesis of AgNPs and zeta potential was evaluated. Morphology and size were analyzed by transmission electron microscope. AgNPs concentrations were determined by atomic absorption spectroscopy. Possibilities of apoptosis induction were confirmed using mitochondrial membrane potential assay, DNA fragmentation assay, and glutathione (GSH) assay.Results: The results indicated that AgNPs were able to induce an inhibition of AMJ13 cells compared their damaging effect toward normal lymphocytes were at minimal according to viability with MTT assay.. Furthermore, these results suggested that AgNPs-induced mitochondrial-mediated apoptosis cause DNA fragmentation, but no significant change in GSH level in AMJ13 cells.Conclusions: The overall results indicated that the physically synthesized AgNPs were exhibited dose-dependent cell death in AMJ13 breast cancer cell line, while the effect of AgNPs on lymphocytes was very low, suggesting that physically synthesized AgNPs might be a potential alternative agent for human breast cancer therapy.