Dissecting T cell lineage relationships by cellular barcoding

Author:

Schepers Koen1,Swart Erwin1,van Heijst Jeroen W.J.1,Gerlach Carmen1,Castrucci Maria2,Sie Daoud3,Heimerikx Mike3,Velds Arno3,Kerkhoven Ron M.3,Arens Ramon1,Schumacher Ton N.M.1

Affiliation:

1. Division of Immunology

2. Istituto Superiore di Sanità, 00161 Roma, Italy

3. Central Microarray Facility, the Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands

Abstract

T cells, as well as other cell types, are composed of phenotypically and functionally distinct subsets. However, for many of these populations it is unclear whether they develop from common or separate progenitors. To address such issues, we developed a novel approach, termed cellular barcoding, that allows the dissection of lineage relationships. We demonstrate that the labeling of cells with unique identifiers coupled to a microarray-based detection system can be used to analyze family relationships between the progeny of such cells. To exemplify the potential of this technique, we studied migration patterns of families of antigen-specific CD8+ T cells in vivo. We demonstrate that progeny of individual T cells rapidly seed independent lymph nodes and that antigen-specific CD8+ T cells present at different effector sites are largely derived from a common pool of precursors. These data show how locally primed T cells disperse and provide a technology for kinship analysis with wider utility.

Publisher

Rockefeller University Press

Subject

Immunology,Immunology and Allergy

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