Affiliation:
1. From the Laboratory of Clinical Investigations and the Laboratory of Immunology, the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda
Abstract
1. The removal of the ß2A-globulins from three sera from treated ragweed-sensitive individuals by immune absorption was associated with the loss of all detectable skin-sensitizing antibody activity as demonstrated by Prausnitz-Küstner testing.
2. Gel filtration studies, with sephadex G-200, indicated that the fractions containing only macroglobulins were devoid of all detectable skin-sensitizing antibody activity.
3. The immune absorption of the γ-globulins from a serum fraction containing ß2A-globulins, γ-globulins, and a trace of ß2M-globulins had no detectable influence on the skin-sensitizing antibody activity.
4. The removal of a portion of the albumin from the allergic sera by immune absorption, with retention of the skin-sensitizing activity, indicated that the loss of skin-sensitizing antibody was not due to non-specific absorption on an antigen-antibody precipitate.
5. No inhibition of the Prausnitz-Küstner reaction was observed when sheep serum, normal human serum, or normal human γ-globulins were tested in concentrations described.
6. We conclude that the skin-sensitizing antibody activities in the three sera from treated ragweed-sensitive individuals studied were associated with the ß2A-globulins.
Publisher
Rockefeller University Press
Subject
Immunology,Immunology and Allergy
Cited by
151 articles.
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