Impaired degranulation but enhanced cytokine production after FcεRI stimulation of diacylglycerol kinase ζ–deficient mast cells

Author:

Olenchock Benjamin A.1,Guo Rishu2,Silverman Michael A.1,Wu Jennifer N.1,Carpenter Jeffery H.2,Koretzky Gary A.13,Zhong Xiao-Ping24

Affiliation:

1. The Signal Transduction Program, The Abramson Family Cancer Research Institute and

2. Department of Pediatrics and

3. The Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104

4. Department of Immunology, Duke University Medical Center, Durham, NC 27710

Abstract

Calcium and diacylglycerol are critical second messengers that together effect mast cell degranulation after allergen cross-linking of immunoglobulin (Ig)E-bound FcεRI. Diacylglycerol kinase (DGK)ζ is a negative regulator of diacylglycerol-dependent signaling that acts by converting diacylglycerol to phosphatidic acid. We reported previously that DGKζ−/− mice have enhanced in vivo T cell function. Here, we demonstrate that these mice have diminished in vivo mast cell function, as revealed by impaired local anaphylactic responses. Concordantly, DGKζ−/− bone marrow–derived mast cells (BMMCs) demonstrate impaired degranulation after FcεRI cross-linking, associated with diminished phospholipase Cγ activity, calcium flux, and protein kinase C–βII membrane recruitment. In contrast, Ras-Erk signals and interleukin-6 production are enhanced, both during IgE sensitization and after antigen cross-linking of FcεRI. Our data demonstrate dissociation between cytokine production and degranulation in mast cells and reveal the importance of DGK activity during IgE sensitization for proper attenuation of FcεRI signals.

Publisher

Rockefeller University Press

Subject

Immunology,Immunology and Allergy

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