QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION

Author:

Heidelberger Michael1,Kendall Forrest E.1,Teorell Torsten1

Affiliation:

1. From the Department of Medicine, College of Physicians and Surgeons, Columbia University, and the Presbyterian Hospital, New York

Abstract

1. A quantitative study has been made of the effect on the precipitin reaction between the specific polysaccharide of Type III pneumococcus and the homologous antibody of salt concentrations ranging from O.1 M to 1.79 M, including the effect of ions of higher valence. 2. Within these limits, observed decreases in precipitated antibody with increasing salt concentration appear to be due to a decrease in the amount of antibody combined with the S III, rather than to an increase in solubility of the S III-antibody compounds. 3. The egg albumin-antibody reaction is far less sensitive to changes in salt concentration than is the S III-antibody reaction.

Publisher

Rockefeller University Press

Subject

Immunology,Immunology and Allergy

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