DEFICIT OF SPECIFIC THYMUS-DEPENDENT LYMPHOCYTES IN TRANSPLANTATION TOLERANCE IN THE RAT

Abstract

Recovery from adoptively terminated transplantation tolerance was studied by utilizing formerly tolerant rats as donors of lymphocytes in local renal graft-vs.-host reactions (GVHR). The origin of the proliferating lymphocytes in the GVHR was studied by means of sex chromosome markers. A deficit of specifically reactive lymphocytes, while tolerance was in effect, was revealed by the continuing absence of autochthonous specifically reactive cells after tolerance was abolished in adult thymectomized chimeras. The findings are consistent with Burnet's hypothesis of the cellular basis of tolerance, but apply only to the T lymphocytes of donor origin which normally proliferate in these GVHR.