Stunning of neutrophils accounts for the anti-inflammatory effects of clodronate liposomes-Reference-Cited by-同舟云学术

Stunning of neutrophils accounts for the anti-inflammatory effects of clodronate liposomes

Published:2023-03-28 Issue:6 Volume:220 Page:
ISSN:0022-1007
Container-title:Journal of Experimental Medicine
language:en
Short-container-title:

Author:

Culemann Stephan¹²^ORCID,Knab Katharina¹²^ORCID,Euler Maximilien¹²^ORCID,Wegner Anja¹²^ORCID,Garibagaoglu Hilal¹²^ORCID,Ackermann Jochen¹²^ORCID,Fischer Kim¹²^ORCID,Kienhöfer Deborah¹²^ORCID,Crainiciuc Georgiana³^ORCID,Hahn Jonas¹²^ORCID,Grüneboom Anika¹²^ORCID,Nimmerjahn Falk⁴^ORCID,Uderhardt Stefan¹²^ORCID,Hidalgo Andrés³⁵^ORCID,Schett Georg¹²^ORCID,Hoffmann Markus H.¹²⁶^ORCID,Krönke Gerhard¹²^ORCID

Affiliation:

1. Department of Internal Medicine 3 - Rheumatology and Immunology, Friedrich-Alexander University Erlangen-Nürnberg and Universitätsklinikum Erlangen 1 , Erlangen, Germany

2. Deutsches Zentrum für Immuntherapie, Friedrich-Alexander University Erlangen-Nürnberg and Universitätsklinikum Erlangen 2 , Erlangen, Germany

3. Area of Cell and Developmental Biology, Centro Nacional de Investigaciones Cardiovasculares Carlos III 3 , Madrid, Spain

4. Institute of Genetics at the Department of Biology, Friedrich-Alexander University Erlangen-Nürnberg 4 , Erlangen, Germany

5. Vascular Biology and Therapeutics Program and Department of Immunobiology, Yale University School of Medicine 5 , New Haven, CT, USA

6. Department of Dermatology, Allergy and Venerology, University of Lübeck 6 , Lübeck, Germany

Abstract

Clodronate liposomes (Clo-Lip) have been widely used to deplete mononuclear phagocytes (MoPh) to study the function of these cells in vivo. Here, we revisited the effects of Clo-Lip together with genetic models of MoPh deficiency, revealing that Clo-Lip exert their anti-inflammatory effects independent of MoPh. Notably, not only MoPh but also polymorphonuclear neutrophils (PMN) ingested Clo-Lip in vivo, which resulted in their functional arrest. Adoptive transfer of PMN, but not of MoPh, reversed the anti-inflammatory effects of Clo-Lip treatment, indicating that stunning of PMN rather than depletion of MoPh accounts for the anti-inflammatory effects of Clo-Lip in vivo. Our data highlight the need for a critical revision of the current literature on the role of MoPh in inflammation.

Funder

Deutsche Forschungsgemeinschaft

Friedrich-Alexander University Erlangen-Nürnberg

Bundesministerium für Bildung und Forschung

European Union

National Institutes of Health

National Institute of Allergy and Infectious Diseases

European Commission

Ministerio de Ciencia e Innovacion

Fundacion La Caixa

Publisher

Rockefeller University Press