THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT-Reference-Cited by-同舟云学术

THE PREPARATION AND PHYSICOCHEMICAL CHARACTERIZATION OF THE SERUM PROTEIN COMPONENTS OF COMPLEMENT

Published:1941-10-01 Issue:4 Volume:74 Page:297-308
ISSN:1540-9538
Container-title:Journal of Experimental Medicine
language:en
Short-container-title:

Author:

Pillemer L.¹,Ecker E. E.¹,Oncley J. L.¹,Cohn E. J.¹

Affiliation:

1. From the Department of Physical Chemistry, Harvard Medical School, Boston, and the Institute of Pathology, Western Reserve University, and the University Hospitals, Cleveland

Abstract

1. Methods for the separation from guinea pig serum in highly purified form of three of the components of complement are described. These substances are the so called mid-piece, end-piece, and 4th component. 2. Mid-piece has been separated as a euglobulin, with an electrophoretic mobility of 2.9 x 10–5 in phosphate buffer of ionic strength 0.2 at pH 7.7, and with a sedimentation constant of 6.4 x 10–13 in potassium chloride of ionic strength 0.2. 3. End-piece and 4th component were found together in a euglobulin fraction of serum which contained 10.3 per cent carbohydrate and had an electrophoretic mobility of 4.2 x 10–5 in phosphate buffer of ionic strength 0.2 at pH 7.7.

Publisher

Rockefeller University Press

Subject

Immunology,Immunology and Allergy

Link

http://rupress.org/jem/article-pdf/74/4/297/1182241/297.pdf

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