A METHOD FOR OBTAINING SUSPENSIONS OF LIVING CELLS FROM THE FIXED TISSUES, AND FOR THE PLATING OUT OF INDIVIDUAL CELLS

Author:

Rous Peyton1,Jones F. S.1

Affiliation:

1. From the Laboratories of The Rockefeller Institute for Medical Research.

Abstract

Individual, living, tissue cells can be obtained in suspension by digesting with trypsin the clot of growing tissue cultures. Under these circumstances the living cells assume a spherical form. When washed and plated in fresh plasma they put out processes and proliferate. After growth in the new plates has occurred the digestion and plating can be repeated. The limits of the method have not yet been reached. We are at work on a number of the problems which it has opened up.

Publisher

Rockefeller University Press

Subject

Immunology,Immunology and Allergy

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