Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAFV600E mutation-Reference-Cited by-同舟云学术

Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAFV600E mutation

Published:2019-04-11 Issue:5 Volume:216 Page:1061-1070
ISSN:0022-1007
Container-title:Journal of Experimental Medicine
language:en
Short-container-title:

Author:

García-Silva Susana¹^ORCID,Benito-Martín Alberto²,Sánchez-Redondo Sara¹,Hernández-Barranco Alberto¹^ORCID,Ximénez-Embún Pilar³,Nogués Laura¹,Mazariegos Marina S.¹,Brinkmann Kay⁴,Amor López Ana¹,Meyer Lisa⁴,Rodríguez Carlos⁵,García-Martín Carmen⁵,Boskovic Jasminka⁵,Letón Rocío⁶,Montero Cristina⁶,Robledo Mercedes⁶,Santambrogio Laura⁷,Sue Brady Mary⁸,Szumera-Ciećkiewicz Anna⁹,Kalinowska Iwona¹⁰,Skog Johan¹¹,Noerholm Mikkel⁴,Muñoz Javier³,Ortiz-Romero Pablo L.¹²,Ruano Yolanda¹³^ORCID,Rodríguez-Peralto José L.¹³,Rutkowski Piotr¹⁰,Peinado Héctor¹^ORCID

Affiliation:

1. Microenvironment and Metastasis Laboratory, Molecular Oncology Program, Spanish National Cancer Research Center, Madrid, Spain

2. Children's Cancer and Blood Foundation Laboratories, Department of Pediatrics, Weill Cornell Medicine, New York, NY

3. Proteomics Unit–ProteoRed-Instituto de Salud Carlos III, Spanish National Cancer Research Center, Madrid, Spain

4. Exosome Diagnostics, GmbH, Martinsried, Germany

5. Electron Microscopy Unit, Spanish National Cancer Research Center, Madrid, Spain

6. Hereditary Endocrine Group, Spanish National Cancer Research Center, Madrid, Spain

7. Department of Pathology, Microbiology & Immunology, Albert Einstein College of Medicine, New York, NY

8. Memorial Sloan Kettering Cancer Center, New York, NY

9. Maria Sklodowska-Curie Institute–Oncology Center, Department of Pathology and Laboratory Medicine, Warsaw, Poland

10. Maria Sklodowska-Curie Institute–Oncology Center, Department of Soft Tissue/Bone Sarcoma and Melanoma, Warsaw, Poland

11. Exosome Diagnostics, Inc., Waltham, MA

12. Department of Dermatology, Medical school, Universidad Complutense de Madrid, Instituto i+12, Hospital Universitario 12 de Octubre, Madrid, Spain

13. Department of Pathology, Medical school, Universidad Complutense de Madrid, Instituto i+12, Hospital Universitario 12 de Octubre, Madrid, Spain

Abstract

Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers of tumor progression in circulating extracellular vesicles could be a powerful non-invasive approach in this setting. We have characterized extracellular vesicles purified from the lymphatic drainage also known as exudative seroma (ES) of stage III melanoma patients obtained after lymphadenectomy. Proteomic analysis showed that seroma-derived exosomes are enriched in proteins resembling melanoma progression. In addition, we found that the BRAFV600E mutation can be detected in ES-derived extracellular vesicles and its detection correlated with patients at risk of relapse.

Funder

National Institutes of Health

Feldstein Foundation

Starr Cancer Consortium

Ministerio de Economía y Competitividad

Ramón y Cajal Program

"La Caixa" Foundation

Fundación Científica Asociación Española Contra el Cáncer

Fundación de Investigación Oncológica

Ministerio de Economía y Competitividad-Red de Excelencia en Investigación e Innovación en Exosomas

Ramón Areces Foundation

Atresmedia-Fundación AXA