Intestinal monocytes and macrophages are required for T cell polarization in response to Citrobacter rodentium

Author:

Schreiber Heidi A.1,Loschko Jakob1,Karssemeijer Roos A.1,Escolano Amelia1,Meredith Matthew M.1,Mucida Daniel1,Guermonprez Pierre12,Nussenzweig Michel C.11

Affiliation:

1. Laboratory of Molecular Immunology, Mucosal Immunology and Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065

2. Laboratory of Phagocyte Immunobiology, Center for Molecular and Cellular Biology of Inflammation, Peter Gorer Department of Immunobiology, Division of Immunology, Infection and Inflammatory Disease, King’s College, London SE5 8AN, UK

Abstract

Dendritic cells (DCs), monocytes, and macrophages are closely related phagocytes that share many phenotypic features and, in some cases, a common developmental origin. Although the requirement for DCs in initiating adaptive immune responses is well appreciated, the role of monocytes and macrophages remains largely undefined, in part because of the lack of genetic tools enabling their specific depletion. Here, we describe a two-gene approach that requires overlapping expression of LysM and Csf1r to define and deplete monocytes and macrophages. The role of monocytes and macrophages in immunity to pathogens was tested by their selective depletion during infection with Citrobacter rodentium. Although neither cell type was required to initiate immunity, monocytes and macrophages contributed to the adaptive immune response by secreting IL-12, which induced Th1 polarization and IFN-γ secretion. Thus, whereas DCs are indispensable for priming naive CD4+ T cells, monocytes and macrophages participate in intestinal immunity by producing mediators that direct T cell polarization.

Publisher

Rockefeller University Press

Subject

Immunology,Immunology and Allergy

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