Estimating the proportion of resistance alleles from bulk Sanger sequencing, circumventing the variability of individual DNA

Author:

Sudo Masaaki1,Yamamura Kohji2,Sonoda Shoji3,Yamanaka Takehiko2

Affiliation:

1. Institute of Fruit Tree and Tea Science, NARO, Kanaya Tea Research Station

2. Institute for Agro-Environmental Sciences, NARO

3. School of Agriculture, Utsunomiya University

Publisher

Pesticide Science Society of Japan

Subject

Health, Toxicology and Mutagenesis,Insect Science

Reference32 articles.

1. 1) F. Gould, A. Anderson, A. Jones, D. Sumerford, D. G. Heckel, J. Lopez, S. Micinski, R. Leonard and M. Laster: Initial frequency of alleles for resistance to Bacillus thuringiensis toxins in field populations of Heliothis virescens. Proc. Natl. Acad. Sci. U.S.A. 94, 3519–3523 (1997).

2. 2) B. E. Tabashnik, A. L. Patin, T. J. Dennehy, Y.-B. Liu, Y. Carrière, M. A. Sims and L. Antilla: Frequency of resistance to Bacillus thuringiensis in field populations of pink bollworm. Proc. Natl. Acad. Sci. U.S.A. 97, 12980–12984 (2000).

3. 3) G. Li, D. Reisig, J. Miao, F. Gould, F. Huang and H. Feng: Frequency of Cry1F non-recessive resistance alleles in North Carolina field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae). PLoS One 11, e0154492 (2016).

4. 4) D. A. Andow and D. N. Alstad: F2 Screen for rare resistance alleles. J. Econ. Entomol. 91, 572–578 (1998).

5. 5) M. Grbić, T. Van Leeuwen, R. M. Clark, S. Rombauts, P. Rouzé, V. Grbić, E. J. Osborne, W. Dermauw, P. C. Thi Ngoc, F. Ortego, P. Hernández-Crespo, I. Diaz, M. Martinez, M. Navajas, É. Sucena, S. Magalhães, L. Nagy, R. M. Pace, S. Djuranović, G. Smagghe, M. Iga, O. Christiaens, J. A. Veenstra, J. Ewer, R. M. Villalobos, J. L. Hutter, S. D. Hudson, M. Velez, S. V. Yi, J. Zeng, A. Pires-daSilva, F. Roch, M. Cazaux, M. Navarro, V. Zhurov, G. Acevedo, A. Bjelica, J. A. Fawcett, E. Bonnet, C. Martens, G. Baele, L. Wissler, A. Sanchez-Rodriguez, L. Tirry, C. Blais, K. Demeestere, S. R. Henz, T. R. Gregory, J. Mathieu, L. Verdon, L. Farinelli, J. Schmutz, E. Lindquist, R. Feyereisen and Y. Van de Peer: The genome of Tetranychus urticae reveals herbivorous pest adaptations. Nature 479, 487–492 (2011).

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